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KMID : 0545120160260091527
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 9 p.1527 ~ p.1532
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Optimization of Hyaluronidase Inhibition Activity from Prunus davidiana (Carriere) Franch Fruit Extract Fermented by its Isolated Bacillus subtilis Strain SPF4211
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Kim Won-Baek
Park So-Hae
Koo Kyoung-Yoon
Kim Bo-Ram
Kim Min-Ji
Lee Hee-Seob
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Abstract
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Strain SPF4211, having hyaluronidase (HAase) inhibition activity, was isolated from P. davidiana (Carriere) Franch fruit (PrDF) sugar extract. The phenotypic and biochemical properties based on 16S rDNA sequencing and an API 50 CHB kit suggested that the organism was B. subtilis. To optimize the HAase inhibition activity of PrDF extract by fermentation of strain SPF4211, a central composite design (CCD) was introduced based on three variables: concentration of PrDF extract (X1: 1?5%), amount of starter culture (X2: 1?5%), and fermentation time (X3: 0?7 days). The experimental data were fitted with quadratic regression equations, and the accuracy of the equations was analyzed by ANOVA. The statistical model predicted the highest HAase inhibition activity of 37.936% under the optimal conditions of X1 = 1%, X2 = 2.53%, and X3 = 7 days. The optimized conditions were validated by observation of an actual HAase inhibition activity of 38.367% from extract of PrDF fermented by SPF4211. These results agree well with the predicted model value.
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KEYWORD
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Prunus davidiana (Carriere) Franch Fruit, HAase inhibition activity, optimization, response surface methodology
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